Currently, the rice-fallow cropping is targeted for intensification in South Asia. Rice-fallow a rain-fed mono-cropping system remain fallow after rice due to lack of irrigation facilities and poor socio-economic conditions of the farmers. Nevertheless, there is scope including winter crops ecological adapt to limited water conditions of the rice-fallow effective moisture conservation practices.
This study aims to identify winter-crops adaptable and productive in the rice-fallow conditions and to evaluate different treatment based practices for water conservation plant formation soil, grain yield, economy and sustainability parameters. Six formation of different plants and management of residues (CERM) practice, namely, zero-tillage featured live rice (ZTDSR), zero-processing transplant rice (ZTTPR), puddled transplanted rice (PTR), ZTDSR with rice residue retention (ZTDSRR +) , ZTTPR with rice residue retention (ZTTPRR +), PTR with rice residue retention (PTRR +) as the main treatment-plots and five of winter crops (beans, lentils, safflower, flaxseed, and mustard) for the treatment of sub-plots were evaluated in split-plot design.
Productivity nuts seeds (beans and lentils) Higher oilseed crops in conditions of rice-fallow sequences Beans> Lentils> safflower> mustard> flaxseed. Among the CERM practices, care ZTDSRR + and ZTDSR increase grain yield of all winter crops on treatment PTR, which is mainly due to the retention of soil moisture is higher for an extended period. increase in grain yield with soil conservation practices that are prevalent in safflower (190%) followed by lentils (93%) and beans (70%).
grain rice yield was higher (7-35%) under treatment followed by treatment ZTDSR PTR. processing conservation practices (ZTDSR, ZTTPR) reduce the use of energy (11-20%) and an increase in energy over conventional tillage practice (PTR), were higher in rice-safflower, rice-lentil and rice-bean rotation. a higher net return achieved in safflower and rice-rice-bean rotation with treatment ZTDSRR +. Predicted greenhouse gas emissions were markedly reduced in the treatment ZTDSR (30%) compared to treatment ZTTPR and PTR.
Therefore, this study suggests that the growing intensification of rice-fallow with the influx of winter crops such as beans, lentils, and safflower follow soil conservation practices (ZTDSRR + in particular) can be a strategic choice to achieve the productivity of the system of higher profits economy, and the use of energy efficiency with reduced emissions of greenhouse gases.
Crop rotation and tillage management options for sustainable intensification of rice-fallow agro-ecosystem in eastern India
[Intensification with high-dose chemotherapy for germ cell tumor, is still the subject of ongoing?]
More than 80% of patients with metastatic germ cell tumor cured with first line chemotherapy. Twenty to 30% of patients will relapse or refractory disease with very poor long-term prognosis. Most of them had metastatic germ cell tumors with a poor prognosis according to a collaborative group of international germ cell classification (IGCCCG).
The role of treatment intensification with high-dose chemotherapy (HDCT) followed by stem cell rescue has not been proven yet in the first line setting compared with standard chemotherapy. HDCT role in the rescue of the first or second has not shown, many studies have been published in this situation with a lot of different regimens.
Description: This product includes one 96-well plate with 96 protein folding solutions, 0.5 ml in each well of the mother plate; 1.4 ml of Inclusion Body Solubilizer; 4 ml of Neutralizer. Each experiment uses 0.1 ml of the solutions from the mother plate. Each mother plate contains 0.5 ml of solutions in each well and can be used for multiple experiments of folding various proteins.
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Description: Rho-associated Kinase (ROCK) mediates Rho signaling and reorganizes the actin cytoskeleton by phosphorylation of several substrates that contribute to the assembly of actin filaments and contractility. ROCK inactivates myosin phosphatase through the specific phosphorylation of myosin phosphatase target subunit 1 (MYPT1) at Thr-696, which results in an increase in the phosphorylated content of the 20-kDa myosin light chain (MLC20). Our 96-Well ROCK Activity Assay Kit uses a safe, non-radioactive format to measure the level of active Rho Kinase in cell or tissue lysates. The kit contains a strip-well plate pre-coated with recombinant MYPT1.
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.
Description: Our OxiSelect 96-Well Comet Assay Kits provide a higher-throughput way to screen for general DNA damage, regardless of the source or nature of the damage. Kits contain Comet Slides, reagents, and a fluorescent dye to visualize cells under an epifluorescence microscope.
PVDF membrane-bottomed 96-well plate + lid (ELISPOT)
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.
Description: The Radius Cell Migration Assay provides a unique alternative to conventional cell migration assays using the Boyden chamber. Unlike Boyden chamber assays which may only be analyzed at endpoint, the Radius assay uses a proprietary cell culture plate containing a carefully-defined biocompatible hydrogel (Radius gel) spot centralized at the bottom of each well. When cells are seeded in the well, they will attach everywhere except on the Radius gel, creating a cell-free zone. Following cell seeding the Radius gel is removed, allowing migratory cells to move across the area and close the gap.
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.
Description: The OxiSelect Comet Assay Slides are useful as a screening tool for various types of DNA damage. Slides are specially treated for adhesion of low-melting agarose. Easily visualize results by epifluorescence microscopy.
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.
Description: Our CytoSelect 96-Well Cell Transformation Assay (Soft Agar Colony Formation) is suitable for measuring cell transformation where no downstream analysis is required. Cells are incubated in a semisolid agar medium for 7-8 days. The cells are then solubilized, lysed and detected using the included fluorescent dye in a fluorometric plate reader.
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.
Description: Phagocytosis can be assayed by measuring the engulfment of a cell "substrate". However, traditional assays require tedious cell counting under a microscope. Our CytoSelect 96-Well Phagocytosis Assay, Zymosan Substrate provides a more accurate, user-friendly, high-throughput alternative to the standard phagocytosis assay. The assay may be adapted for use with 24-well or 48-well plates.
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.
Description: Our 96-well Checkpoint Kinase Activity Assay Kit provides a non-isotopic, sensitive and specific method to monitor checkpoint kinase activity using its physiological substrate; it can also be used in screening checkpoint kinase inhibitors.
Description: Hematopoietic stem cells (HSCs) are well-characterized, tissue-specific stem cells that are responsible for the lifelong maintenance of the hematopoietic system. HSCs or hematopoietic progenitors known as colony-forming cells (CFCs) proliferate to form discrete colonies when cultured in a suitable 3D environment, such as methylcellulose supplemented with nutrients and cytokines. Our CytoSelect 96-Well Hematopoietic Colony Forming Cell Assay promotes the formation of HSC colonies in just 7-10 days. Cells can then be either quantified in a fluorescence plate reader or recovered from the semisolid medium for further downstream analysis.
Description: Adhesion to the extraceullular matrix is essential for the survival and propagation of many adherent cells. Apoptosis resulting from the loss of adhesion to the ECM is known as anoikis. Anoikis is involved in the physiological processes of tissue renewal and cell homeostasis. Our CytoSelect Anoikis Assays allow you to quantify and monitor anoikis in cells using a precoated plate. Live cells can be viewed under a microscope and quantified on a plate reader by MTT (colorimetric) or Calcein AM (fluorometric); both reagents are included in the kit. Dead cells are detected with the red EthD-1 reagent, also included.
Outside of clinical trials, HDCT remain an option in salvage therapy, depending on many factors including prognostics factors, previous therapy, the general conditions and consideration of referral centers for selecting eligible patients who can benefit most of these approaches. The results of TIGER international randomized trial will provide evidence-based information for HDCT strategy.
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