plant-microbe interactions in the rhizosphere influence the level of mineralization of organic matter and nutrient cycling that is essential for sustainable agricultural productivity. intensification of agriculture, particularly the introduction of synthetic fertilizer in the United States, changed the abundance and dominant forms of nitrogen (N), essential plant nutrients, potentially impose selection pressure on the properties of plants and plant-microbe interactions that regulate N cycling and acquisitions.
We hypothesize that the adaptation of corn to N fertilization synthetic functional properties and altered root rhizosphere microbial nutrient cycles, reducing the ability of corn to obtain N from organic sources. Six inbreds released pre-fertilizer (1936, 1939, 1942) or post-fertilizer (1984, 1994, 2015) were grown in rhizoboxes 15N-labeled blots containing clover / vetch residue. multivariate approach does not identify the characteristics of a strong and consistent architecture predictable rhizosphere processes, although the morphological plasticity root metrics related to carbon-and N-cycling enzyme activity.
the characteristics of the root, the activities of potential extracellular enzymes (BG, LAP, NAG, urease), the abundance of N-cycling genes (Amoa, narG, nirK, NIR, nosZ) and the absorption of organic N did not differ between the era of the release despite substantial variation among genotypes and replications. Thus, agricultural intensification does not appear to have impaired N cycling and acquisition from organic sources by modern corn and its rhizobiome. Increased mechanistic understanding of rhizosphere processes and their response to selective pressures would contribute greatly to the rhizosphere engineering for sustainable agriculture.
double staining protocol using most popular immunoperoxidase technique can increase the difficulty. Both regular detection system may be cross-talk, when the primary antibodies derived from animals closely related phylogenetically. Color shift polymer 3,3′-diaminobenzidine (DAB) may occur during the second development, which resulted in a poor distinction between the two types of deposits.
A post-DAB technique, intensification-silver-gold sulfide, fine tuned to eliminate these difficulties, that may be very suitable for the cell nucleus and perikarya colocalization of the same cells. The revised method was examined in combination with the other steps subsequent immunoperoxidase or fluorochrome-tagged reagents. Nuclear Antigen (BrdU, c-Fos, and Prox-1) was first visualized with DAB polymer, which is then treated with SSGI, rotating black deposit.
Has agricultural intensification impacted maize root traits and rhizosphere interactions related to organic N acquisition?
Present and Future of de-intensification Strategies in the Treatment of Oropharyngeal Carcinoma
Purpose of review: The treatment of patients with squamous cell carcinoma of the oropharynx (OPSCC) is still controversial. Positive HPV is widely accepted as a favorable prognostic factor, and HPV + OPSCC regarded as different pathological entities with NCCN guidelines are dedicated and probably deserved more personalized treatment strategies.
The possibility to reduce invasive surgical and acute and late toxicity of radiotherapy / chemotherapy has led to a new concept of treatment of de-escalation strategy. In particular, some de-intensive approaches have been investigated with the aim of giving the patient a less toxic treatments, while maintaining comparable results in terms of disease control and survival.
Description: A sandwich quantitative ELISA assay kit for detection of Human Immunoglobulin D (IgD) in samples from serum, plasma or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Human Immunoglobulin D (IgD) in samples from serum, plasma or other biological fluids.
Description: A Mouse Monoclonal antibody against Myosin Heavy Chain from Human/ Mouse/ Rat/ Fruit Fly/ Nematode. This antibody is tested and validated for IHC, IF
Description: A Mouse Monoclonal antibody against Myosin Heavy Chain from Human/ Mouse/ Rat/ Fruit Fly/ Nematode. This antibody is tested and validated for IHC, IF
Description: A Rabbit Polyclonal antibody against Ferritin heavy chain from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A Rabbit Polyclonal antibody against Ferritin heavy chain from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A polyclonal antibody for detection of Ferritin heavy chain from Human, Mouse, Rat. This Ferritin heavy chain antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Ferritin heavy chain
Description: A polyclonal antibody for detection of Ferritin heavy chain from Human, Mouse, Rat. This Ferritin heavy chain antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Ferritin heavy chain
Description: A polyclonal antibody for detection of Ferritin heavy chain from Human, Mouse, Rat. This Ferritin heavy chain antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human Ferritin heavy chain
Description: A monoclonal antibody for detection of Myosin Heavy Chain from Human, Mouse, Rat, Fruit Fly, Nematode. This Myosin Heavy Chain antibody is for IF. It is affinity-purified from mouse ascites by affinity-chromatography using the specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide
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The purpose of this review was to systematically describe the status of research in the de-intensification strategy of surgical and non-surgical in the OPSCC treatment.
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